KMID : 0878220090210030138
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Journal of Sasang Constitutional Medicine 2009 Volume.21 No. 3 p.138 ~ p.153
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Protection effect of New-Yeolda-Hanso tang against ¥â-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells
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Bae Na-Young
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Abstract
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1.Objectives: Yeolda-Hanso tang (YH) has long been used as traditional herbal formula in Korea as various diseases. Now we modified Yeolda-Hanso tang (YH) for neurodegenerative diseases treatment and named New-Yeolda-Hanso tang (NYH). We investigated neuroprotective effects of NYH on NGF-differentiated PC12 cells cytotoxicity induced by ¥â-Amyloid peptide (A¥â25-35) and evaluated the ability of NYH to prevent and treat for neurodegenerative diseases via autophagy enhancement.
2.Methods and Results: 1) Protective effect of NYH on PC12 cells cytotoxity induced by A¥â25-35. PC12 cells survival was measured by MTT and lactate dehydrogenase (LDH) assay. 20¥ì¥Ì ¥â-Amyloid peptide (A¥â25-35) induced cytotoxicity on NGF-differentiated PC12 cells. NYH attenuated the cytotoxic effects of A¥â25-35 in a dose-dependent manner. 2) Pharmacological induction of Autophagy by NYH in PC12 cells Autophagy induction and activation was measured by immunoblot assay. Marker of autophagy, LC3¥± expression and the ratio of LC3-II/I was slightly increased in the protein treated with YH, and significantly augmented in the protein treated with NYH. NYH-induced increase of LC3-II protein level was inhibited by 3MA. 3) Induction of Autophagy by NYH on A¥â25-35-induced injury in PC12 cells In MTT assay, 100§¶/§¢ pre-treated NYH attenuated 20¥ì¥Ì A¥â25-35-induced cytotoxicity in PC12 cells. Protection effect of NYH was blocked by autophagy inhibitor 3MA. In immunoblot assay, 1200§¶/§¢ pre-treated NYH activated autophagy in 20¥ì¥Ì A¥â25-35-induced cytotoxicity in PC12 cells. The observed effect was partially blocked by 3MA.
3.Conclusions: All the results indicated that NYH possesses neuroprotective potential partially mediated by autophagy enhancement and NYH may be considered to be a promising new herbal formula to prevent and treat for neurodegenerative diseases including Alzheimer¡¯s disease (AD).
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KEYWORD
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New-Yeolda-Hanso tang (NYH), Neuroprotection, Alzheimer¡¯s disease, ¥â-Amyloid, Autophagy
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